Yesterday, my day started out cleaning cat pee off the floor.
Then I tried to use up all the open bananas from yesterday, but I didn’t have time to cook everything before work so some will rot in the fridge.
Then I got to work to find we had more samples than we’d hoped, and we also had to drive to a lab in Cambridge to do our work because a piece of very important equipment here is dead.
Then we got rejected by a possible guest for the convention.
Then we had to go through orientation and stuff at the other lab (fair enough, we’ll be performing work there, after all) and that coupled with the disorientation caused by doing wok in a corner of a borrowed lab meant that our normal extraction took until 2 (instead of noonish like normal).
Then we had to wait for our other reagents to thaw, since we were not here to take them out of the freezer earlier.
Then we had to wait for a salad spinner to be purchased as a band-aid solution to a relatively minor problem caused by a large problem (see previous statements re: important piece of equipment being dead).
Finally got samples into the machine to run, and since it was 3:30 I forgot I hadn’t eaten lunch yet and sat around until 4 wondering why I was so grumpy.
Ate my lunch at 4. It was very tasty.
Samples finished their run at 5:15, and I get to find that half of them had failed. Super.
Then I had to re-run the failed samples, and finally got out of work at quarter to 6.
Today, my day started out cleaning up cat pee off the floor.
Then I got to work and found that 95% of the samples that failed yesterday had also failed in the second run overnight.
So I am thawing reagents to run them again.
I only have 6 samples to test so far today, which would be amazingly helpful, since I have a smaller replacement for the dead piece of lab equipment that I can use to do a small run here quickly and easily… if all of our other supplies weren’t in the other lab already.
I am not overly optimistic for the rest of today.
Freeze the banana’s! Make banana bread later!
Also: Replacing centrifuge with salad spinner = Epic Win.
I can’t eat wheat for the moment, so banana bread is out. I’ve been making banana + egg = “pancake”.
Centrifuge: $13,000
Salad spinner: $7.99
Works in a pinch to get liquids to the bottom of tubes.
Don’t tell my GM or else I’ll have to get all my future lab supplies at the grocery store too. 😉